In order to investigate soil seasonal gleization process in the alpine meadow on the Tibetan plateau, the soil gleization and its two main controls (high soil organic carbon content (SOC) content and anaerobic environment) were reported in the alpine grassland from Dec. 2013 to March 2014. The results show that the soil seasonal gleization occurres in the humus layer with blue-gray iron and manganese plaques. The soil seasonal gleization occures during the period of soil freezing and thawing, and a positive relationship between the thickness of gleization layer and frozen layer is observed. The process is pseudogley at mild level, with decreasing soil microbial biomass carbon, and increasing cation exchange capacity (CEC), active Fe²⁺, Mn²⁺ and availble phosphuros. The reults suggest that soil gleization may subsequently affect microbial community, root mortality, greenhouse gas emmission and nutrient cycling. Although soil gleization appears in the non-growing season, its impacts on the soil ecological processes should not be ignored.